Saan tɛmɛninw na, bana sɛgɛsɛgɛli fɛɛrɛ min be kɛ ni in vitro ye, o yiriwali teliyara minkɛ, kemiluminescent immunoassays (CLIA) kɛra baarakɛminɛn nafaman ye kɛnɛyako sɛgɛsɛgɛli la k’a sababu kɛ u ka sensitiviteba ye, u ka sɛgɛsɛgɛli yɔrɔba ani u ka otomatiki nafaw kosɔn. Komi CLIA sisitɛmu yɔrɔ jɔnjɔn lo, dɛmɛfɛnw ka fɛɛrɛw be nɔɔ to sɛgɛsɛgɛri jaabiw ka tiɲɛn n’u ka lannaya kan. Nin barokun bena sɛgɛsɛgɛri kɛ fɛɛrɛ jɔnjɔnw kan minw be sɔrɔ kemiluminesɛnti immunoassay la ka bɔ yɔrɔ caaman na.
Sensibiliteba ani lɔnniya dan min ka dɔgɔ
Nafaba min be sɔrɔ kemiluminisɛnsi fɛnɲɛnamafagalanw na, o ye u ka tagamasiɲɛw cayacogo la min basiginin be anzimatiki ka yɛlɛmaniw kan wala kemiluminisansi tilennin kan. Ni an ye fɛnw ni tagamasiɲɛw fara ɲɔgɔn kan ka ɲɛ, bi ka fɛnw be se ka femtogramu-nivo (fg/mL) wala hali attogramu -nivo (ag/mL) lɔnniya lɔnniya sɔrɔ, o min be lɔnniya setigiya fisaya kosɔbɛ low{ce {ker, econcentration for-s . i n’a fɔ kansɛri tagamasiɲɛw ani banakisɛw kɛlɛlanw.
Kɛrɛnkɛrɛnninba ani -ɲɔgɔndan kɛlɛli dilan cogo
Walisa ka cross-reactions bali, monoklonal anticorps walima antigène epitope caman siricogo fɛɛrɛw be kɛ reactifs yiriwali la walisa ka se ka sɛgɛsɛgɛlikɛla laɲininw lɔn cogo kɛrɛnkɛrɛnnin na. O wagati kelen na, n’an be furaw fara a kan wala n’an be buffer système fisaya, fɛɛn minw be se ka don mɔgɔ farikolo la (i n’a fɔ joli bɔli ani lipemi) olu ka nɔɔ be se ka dɔgɔya koɲuman jaabiw kan, o la, kɛnɛyako sɛgɛsɛgɛrifɛnw be se ka kɛ ka ɲɛ.
Basigi ani baarakɛcogo nɔgɔya
Reagent ka basigi wagatijan-o lo be se ka kɛ sababu ye ka sɛgɛsɛgɛli kɛ ka bɛn ɲɔgɔn ma. Sisan, fɛɛrɛba min be yen, o be baara kɛ ni mugu jalen ye min be se ka mara degere 2-8 la wala so kɔnɔ kalo 12-24 kɔnɔ. Reactif kariti minw labɛnna ka kɔn, olu ka tɔgɔba be baarakɛcogo nɔgɔya tugun, ani ka baara kɛ ni minɛnw ye minw be baara kɛ u yɛrɛ ma walisa ka se ka "sample in, result out" lɔn cogo ɲuman na.
Ɲɛyirali caman ani mɔgɔ yɛrɛ ka yɛlɛmaniw
Walisa ka se ka dɔgɔtɔrɔso ka laɲiniw dafa, o min ye ka sɛgɛsɛgɛli kɛ ni sɛgɛsɛgɛli ye min be se ka kɛ ni fanga ye, fura dɔw ye dɛmɛ don ka sɛgɛsɛgɛli kɛ ni sɛgɛsɛgɛli ye min be kɛ ni sɛgɛsɛgɛlikɛlan caaman ye, ani u be se ka baara kɛ ni fɛn caaman ye ɲɔgɔn fɛ sɛgɛsɛgɛli kelen kɔnɔ. Ka fara o kan, ka bana minw be bɔra ka na, olu ka sɛgɛsɛgɛli teliman makoya jaabi (i n’a fɔ koronawirisi sugu kuraw), sɛgɛsɛgɛli ni yiriwali min be kɛ ni furakisɛw ye, o be tagara ka taga modularizasiyɔn ni jaabi teliman fan fɛ.
Fɛɛrɛ minw be se ka mɔgɔw farikolo tanga, olu be se ka sɛgɛsɛgɛri kɛ ni furakisɛw ye minw be se ka mɔgɔw farikolo tanga bana ma ani u be se ka baara kɛ koɲuman. Ni minanw lɔnniya ni biyɔ-ɛnzeniyɛri yiriwara, yɔrɔba bele be yen ka yɛlɛmani don musakaw kɔlɔsili la, lɔnniya teliya la ani fɛnw lɔnniya la minw be se ka yɛlɛma yɛlɛma ni hakili ye sini ma.